phenol SULFURIC ACID
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Country Of Origin : India
manufacturing and exporters of phenol
The Phenol-Sulfuric Acid method is a widely used colorimetric assay for the quantitative determination of carbohydrates, including simple sugars and complex polysaccharides. Here’s a breakdown of what it is and how it works:
Applications
Food industry (e.g., sugar content in beverages)
Biochemistry (e.g., analysis of glycogen or starch)
Environmental science (e.g., carbohydrate content in water samples)
Biofuel research (e.g., quantifying sugars in biomass)
manufacture and exports phenol sulfuric acid
Phenol-Sulfuric Acid Method Overview
Principle:
Carbohydrates are dehydrated by concentrated sulfuric acid to form furfural derivatives (e.g., hydroxymethylfurfural from hexoses).
These furfural derivatives react with phenol, producing a yellow-orange color.
The intensity of the color is proportional to the carbohydrate concentration and can be measured spectrophotometrically, usually at 490 nm.
🧪 Reagents:
Phenol solution (5% or 6% w/v in water)
Concentrated sulfuric acid (H₂SO₄)
Standard sugar solution (e.g., glucose or sucrose)
🧫 Protocol (simplified)
